The introduction of FISH in the late 1980s, as a technique that can readily detect trisomies and translocations in metaphase spreads and interphase nuclei using entire chromosome-specific DNA libraries, was heralded as a further revolution in cytogenetic analysis (Pinkel, 1988; Kearney, 2001).

 

Fluorescence, the most commonly used technique in situ hybridization (FISH) detects the presence or absence and location of specific gene sequences found in genetics abnormalities such as genetic diseases.